To have the best results when looking for nipples hold the pup so there is a slight glare from a light such as a lamp on their belly and gently move the pup around so the glare moves among the tummy. You should never pick up a mouse solely by the tail lifting all feet off a surface. Therefore, it is wise to keep track of the mice with and without nipples, if you rely heavily on the nipple method. Noting the difference between the penile shaft and the vaginal orifice is the best method of sexing. When the reflection goes over the nipples, you should be able to see them. If you are unsure of sexing, the following method may help you. Always remember that tails are not handles placed there for the convenience of humans.
Alternatively, you may be able to hold the pup upside down in your hand. Anything with a clear bottom will work. To assist you with sexing your own pups I have provided pictures from ages 3 days old until 23 days old below. Please do not reproduce in any way. Last edited by Peachy on Wed 13 Dec , 8: You can't risk a buck in with your does or a doe in with your bucks. Sexing Adult mice using the Genital method. Sexing Juvenile mice using the Nipple method Standard age. You may notice at this point that the buck in these images is angora. This can result in unwanted babies which could prove devastating to the other and her young. They can snap their back causing a mouse to become paralyzed, or worse, death , break their tail, the skin can come completely off the tail, and their tail can actually break completely off as well mouse tails do not grow back like some lizards. It is better to ask for help than have accidents happen. While some may be able to sex mice well, they are notorious for miss-sexing mice. You will have the best luck finding the nipples farthest back, by the genitalia. On the doe you can see her Vaginal Orifice, behind the Urethral Orifice the "flap of skin" between the anus and nipples. Hold the tank above your head, preferably resting on a secure surface to prevent it from falling. Note that with some lines the bucks and does have less of a anogenital distance gap between the anus and genitals difference, especially in youth. Males will not have nipples. You can even take bum pictures and post them to reputable mouse groups for assistance. Always remember that pinkies can get cold extremely fast. Do note that males can hold their testicles in for long periods of time, therefore looking from below for testicles is not a guarantee you have sexed them properly unless you actually see testicles drop. Better safe than sorry. You should never pick up a mouse solely by the tail lifting all feet off a surface. If you do not have a tank, you can use a glass dish, drinking glass, jar no lid! This is more obvious in older mice who are fully developed. Set up 3 cages; one for the females, one for the males, and one for the mice you are not positive of sex. When the reflection goes over the nipples, you should be able to see them.
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